CD81 antibody

Catalog No. ABIN119111

The Hamster Monoclonal anti-CD81 antibody has been validated for FACS, WB, IP, IHC (fro) and EIA. It is suitable to detect CD81 in samples from Mouse. There is 1 publication available.

Quick Overview for CD81 antibody (ABIN119111)

Target: CD81

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: Eat2

Product Details anti-CD81 Antibody

Purification: Affinity Chromatography on Protein G.

Immunogen: 38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.

Isotype: IgG

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD81 antibody (ABIN119111)

Blumenthal, Giebel, Ummanni, Schlüter, Endlich, Endlich: "Morphology and migration of podocytes are affected by CD151 levels." in: American journal of physiology. Renal physiology, Vol. 302, Issue 10, pp. F1265-77, (2012) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 / TAPA1

Background: TAPA1, or CD81, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterised by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TAPA1 is a cell surface glycoprotein that is known to complex with integrins. It appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. TAPA1 gene is localized in the tumor suppressor gene region and thus it is a candidate gene for malignancies.Synonyms: 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1

Gene ID: 10090

UniProt: P35762

Pathways: Inositol Metabolic Process, Hepatitis C