CD147 antibody

Catalog No. ABIN119252

The Mouse Monoclonal anti-CD147 antibody has been validated for WB, FACS, IF and IP. It is suitable to detect CD147 in samples from Human. There is 1 publication available.

Quick Overview for CD147 antibody (ABIN119252)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: MEM-M6-1

Product Details anti-CD147 Antibody

Specificity: This antibody recognizes the Human CD147 cell surface antigen, a 50-60 kDa glycoprotein also known as neurothelin and basigin.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: CD147Rg, consisting of the cDNA coding for the hinge region, CH2 and CH3 domains of Human IgG1.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 106 cells. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives, None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CD147 antibody (ABIN119252)

Schneiderhan, Scheler, Holzmann, Marx, Gschwend, Bucholz, Gress, Seufferlein, Adler, Oswald: "CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models." in: Gut, Vol. 58, Issue 10, pp. 1391-8, (2009) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147

Background: CD147 is a 50-60kD glycoprotein also known as neurothelin and basigin. CD147 is broadly expressed on peripheral blood cells and by endothelial cells. Expression upon T cells is increased following activation.Synonyms: 5F7, BSG, Basigin, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor

Gene ID: 682

NCBI Accession: NP_001719

UniProt: P35613

Pathways: S100 Proteins