CD8 antibody (FITC)

Catalog No. ABIN119507

The Mouse Monoclonal anti-CD8 antibody has been validated for FACS. It is suitable to detect CD8 in samples from Human.

Quick Overview for CD8 antibody (FITC) (ABIN119507)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: LT8

Product Details anti-CD8 Antibody (FITC)

Specificity: This antibody recognises the CD8 cell surface glycoprotein.

Purification: Ion Exchange Chromatography.

Immunogen: Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

NCBI Accession: NP_001759

UniProt: P01732