CD21 antibody

Catalog No. ABIN119512

This Mouse Monoclonal antibody specifically detects CD21 in FACS, IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD21 antibody (ABIN119512)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LB21

Product Details anti-CD21 Antibody

Purification: Ion exchange chromatography

Immunogen: Human IM9 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended positive control tissue: Lymph node.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 25 µg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background: CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.Synonyms: C3DR, C3d receptor, CR2, Complement C3d receptor, Complement receptor type 2, Dendritic Cell Marker, EBV Receptor, Epstein-Barr virus receptor

Gene ID: 9606

UniProt: P20023

Pathways: Complement System