HLA-A2 antibody (FITC)

Catalog No. ABIN119576

The Mouse Monoclonal anti-HLA-A2 antibody is suitable to detect HLA-A2 in samples from Human. It has been validated for FACS.

Quick Overview for HLA-A2 antibody (FITC) (ABIN119576)

Target: HLA-A2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Clone: BB7-2

Product Details

Specificity: This antibody recognises the HLA-A2 histocompatability antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.

Purification: Affinity chromatography on Protein G

Immunogen: Papain solubilised HLA-A2. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (Neat - 1/10): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood, this antibody may be used for the flow cytometric detection of HLA-A2 expression.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 1 % Bovine Sodium Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: HLA-A2

Alternative Name: HLA Class I A-2 alpha / HLA-A2

Target Type: Influenza Protein

Background: Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2

Gene ID: 100507703

NCBI Accession: XP_003119274

UniProt: P01892