The Mouse Monoclonal anti-CD84 antibody (Clone CD84-1-21) (ABIN119619) specifically detects CD84 in FACS and IP.
The antibody is reactive with Human samples.
This antibody recognises the CD84 cell surface antigen, a highly glycosylated cell surface antigen of 64-82 kDa.
Purification
Affinity chromatography on Protein G
Immunogen
Mouse pre-B cell line 300.19 transfected with CD84 cDNA. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
CD84
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow cytometry: 1/100 - 1/200, use 10 μL of the suggested working dilution to label 10^6 cells. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
CD84
Alternative Name
CD84 / SLAMF5
Background
CD84 is predominantly expressed by B lymphocytes, monocytes and macrophages, although lower levels of expression have also been reported on T cells, granulocytes and platelets.Synonyms: Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, MAX.3, SLAM family member 5, Signaling lymphocytic activation molecule 5