LY9 antibody (PE)

Catalog No. ABIN119625

The PE-conjugated Mouse Monoclonal anti-LY9 antibody (Clone HLy 9-1-25) (ABIN119625) specifically detects LY9 in FACS. The antibody is reactive with Human samples.

Quick Overview for LY9 antibody (PE) (ABIN119625)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: HLy 9-1-25

Product Details anti-LY9 Antibody (PE)

Specificity: This antibody recognises the CD229 cell surface antigen, a 100-120 kDa cell surface glycoprotein that is the homologue of Murine Ly-9.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Mouse pre-B cell line 300.19 transfected with CD229 cDNA. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA, 5 % Sucrose as stabilizers

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: RT/4 °C/-20 °C

Storage Comment: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229 / LY9

Background: CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9

Gene ID: 4063

NCBI Accession: NP_002339

UniProt: Q9HBG7

Pathways: BCR Signaling