MCAM antibody (N-Term)

Catalog No. ABIN119671

This Mouse Monoclonal antibody specifically detects MCAM in FACS, IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for MCAM antibody (N-Term) (ABIN119671)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: OJ79c

Product Details anti-MCAM Antibody

Binding Specificity: AA 1-5, N-Term

Specificity: This antibody recognizes the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumor progression.

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 106 cells. Immunohistochemistry on frozen sections. Recommended positive control tissue: Melanoma. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, None, None

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C only.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to tumour progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Gene ID: 4162

NCBI Accession: NP_006491

UniProt: P43121