MCAM antibody (N-Term) (FITC)

Catalog No. ABIN119672

The Mouse Monoclonal anti-MCAM antibody has been validated for FACS, IHC (fro) and EIA. It is suitable to detect MCAM in samples from Human and Pig. There is 1 publication available.

Quick Overview for MCAM antibody (N-Term) (FITC) (ABIN119672)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: OJ79c

Product Details anti-MCAM Antibody (FITC)

Binding Specificity: AA 1-5, N-Term

Specificity: This antibody recognises the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells.

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant Human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Clone OJ79c (purified antibody) has been successfully used in ELISA and immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MCAM antibody (FITC) (ABIN119672)

Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to disease progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Gene ID: 4162

NCBI Accession: NP_006491

UniProt: P43121