CD177 antibody

Catalog No. ABIN119765

The Mouse Monoclonal anti-CD177 antibody has been validated for WB, FACS and IP. It is suitable to detect CD177 in samples from Human and Rhesus Monkey.

Quick Overview for CD177 antibody (ABIN119765)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: MEM-166

Product Details anti-CD177 Antibody

Specificity: This antibody recognizes CD177 (Neutrophil Glycoprotein NB1). Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). MEM-166 recognizes CD177 under Non-Reducing Conditions.

Purification: Affinity Chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (1/50 - 1/100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western blot: Non-reducing conditions. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population.Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1

Gene ID: 57126

NCBI Accession: NP_065139

UniProt: Q8N6Q3