IGF2R antibody

Catalog No. ABIN119771

The Mouse Monoclonal anti-IGF2R antibody has been validated for WB and FACS. It is suitable to detect IGF2R in samples from Human and Mouse.

Quick Overview for IGF2R antibody (ABIN119771)

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF2R antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: MEM-238

Product Details anti-IGF2R Antibody

Specificity: The antibody recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10 % in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Purification: Affinity Chromatography on Protein-A

Immunogen: Recombinant Vaccinia virus encoding CD222

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Western Blotting under non reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 containing 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -70 °C for longer.

Target Details for IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Alternative Name: CD222 / IGF2R

Background: CD222 is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10 % of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-b, last but not least CD222 serves as a receptor for heparanase and even for Listeria.Synonyms: 300 kDa mannose 6-phosphate receptor, CI Man-6-P receptor, CI-MPR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, Late Endosome Marker, M6P/IGF2 receptor, M6P/IGF2R, M6PR, MPR 300, Mannose 6 Phosphate Receptor (Cation independent)

Gene ID: 3482

NCBI Accession: NP_000867

UniProt: P11717