This antibody recognises CD150, also known as signalling lymphocyte activation molecule (SLAM). Clone 9D1 is reported to have functional activity. We recommend the use of SM2026A for this purpose. This antibody is routinely tested on mouse thymocytes.
Purification
Affinity chromatography on Protein G
Immunogen
CHO cells stably transfected with mouse CD150. Spleen cells from immunised rats were fused with cells of a myeloma cell line.
SLAMF1
Reactivity: Human
FACS
Host: Mouse
Monoclonal
SLAM-4
PE
Application Notes
Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
SLAMF1
(Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
Alternative Name
CD150 / SLAMF1
Background
Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and has been shown to be upregulated on activated T-cells and stimulated macrophages. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T-cells and Bcells.Synonyms: CDw150, Ipo-3, Signaling lymphocytic activation molecule, Slam