SLAMF1 antibody

Catalog No. ABIN1027681

Product Details anti-SLAMF1 Antibody

Target: SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLAMF1 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The mouse monoclonal antibody SLAM.4 recognizes CD150, a cell surface molecule expressed on lymphocytes and involved in their activation.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Sterility: 0.2 μm filtered

Immunogen: Human CD150-transfected 300.19 cells

Clone: SLAM-4

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μ,m filter sterilized.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-SLAMF1 antibody (ABIN1027681)

Romero, Zapater, Calvo, Kalko, de la Fuente, Tovar, Ockeloen, Pizcueta, Engel: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).

Romero, Benítez, March, Vilella, Miralpeix, Engel: "Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4)." in: Tissue antigens, Vol. 64, Issue 2, pp. 132-44, (2004) (PubMed).

Target Details for SLAMF1

Target: SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))

Alternative Name: CD150 (SLAMF1 Products)

Synonyms: CD150 antibody, SLAM antibody, SLAMF1 antibody, 4933415F16 antibody, AA177906 antibody, CDw150 antibody, ESTM51 antibody, IPO-3 antibody, Slam antibody, RGD1560634 antibody, signaling lymphocytic activation molecule family member 1 antibody, SLAMF1 antibody, Slamf1 antibody

Background: CD150, also known as SLAM (signaling lymphocyte activation molecule) is a 70-95 kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leucocyte activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.