IL-10RA antibody

Catalog No. ABIN119864

The Rat Monoclonal anti-IL-10RA antibody has been validated for FACS. It is suitable to detect IL-10RA in samples from Mouse.

Quick Overview for IL-10RA antibody (ABIN119864)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10RA antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 1B1-3a

Product Details anti-IL-10RA Antibody

Specificity: This antibody recognises CD210, a cell surface glycoprotein of approximately 110KD, also known as Interleukin-10 Receptor (IL-10R). The epitope recognised by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CD210. This clone is reported to neutralize the effects of IL-10 (1). SM2046A is recommended for Functional Studies.

Purification: Affinity Chromatography on Protein G

Immunogen: Purified recombinant epitope tagged extracellular region of mouse CD210. Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1/10-1/100): Use 10 μL of the suggested working dilution to label 1x 10^6 cells in 100 μL. Due to low expression of CD210, sensitive staining techniques may be required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: CDw210a / IL10RA

Background: Mouse CD210 is expressed by a range of cells including thymocytes, T cells, B cells and monocytes.Synonyms: IL-10R-A, IL10 Receptor alpha, Interleukin-10 receptor alpha chain

Gene ID: 16154

NCBI Accession: NP_032374

UniProt: Q61727

Pathways: Growth Factor Binding