LAIR1 antibody

Catalog No. ABIN119871

Product Details anti-LAIR1 Antibody

Target: LAIR1 (Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAIR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: This antibody recognises leukocyte-associated Ig-like receptor-1 (LAIR-1). Clone NKTA255 is reported to inhibit NK cell triggering via CD16 Molecules (See Poggi, A. et al.for details). We recommend the use of Azide Free Antibody Cat.-No SM2048A for this purpose. see Poggi, A. et al. for details.

Purification: Affinity Chromatography on Protein G

Immunogen: NK cells lines B12.100 and AM.25. Spleen cells from immunised mice were fused with cells of the P3U1 myeloma cell line.

Clone: NKTA255

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Western Blot: this antibody detects a band of approximately 40 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LAIR1

Target: LAIR1 (Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1))

Alternative Name: CD305 / LAIR1 (LAIR1 Products)

Background: LAIR-1 is a 40kD cell surface molecule that functions as an inhibitory receptor on natural killer cells, monocytes and T cells. LAIR-1, otherwise known as CD305, is also expressed by other haematopoeitic cells including B cells, but is not expressed by nonhaematopoietic, platelets or erythrocytes.Synonyms: LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1, hLAIR1

Gene ID: 3903

NCBI Accession: NP_002278

UniProt: Q6GTX8