FASL antibody

Catalog No. ABIN120319

Product Details anti-FASL Antibody

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody recognises the CD178 cell surface antigen. Clone 14C2 is not reported to have a blocking function. We recommend the use of SM2268LE for functional studies.

Purification: Affinity Chromatography on Protein G

Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.

Clone: 14C2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/25 - 1/50, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Results maybe enhanced using membrane permeabilisation. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand (FASL Products)

Background: CD178 is a 40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Gene ID: 356

NCBI Accession: NP_000630

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity