Factor VIII antibody

Catalog No. ABIN120341

The Mouse Monoclonal anti-Factor VIII antibody has been validated for WB, RIA and EIA. It is suitable to detect Factor VIII in samples from Human and Pig.

Quick Overview for Factor VIII antibody (ABIN120341)

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Factor VIII antibody is un-conjugated

Application: Western Blotting (WB), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: RFFVIIIC-8

Product Details anti-Factor VIII Antibody

Specificity: Clone RFFVIIIC/8 is a very potent coagulation inhibitor. This antibody recognizes an epitope towards the N-terminus of full length Factor VIII. It also recognises the 210 kDa, 90 kDa and 40 kDa cleavage products. This clone does not cross-react with von Willebrand factor.

No Cross-Reactivity: Mouse (Murine), Dog (Canine), Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Human and Pig.

Purification: Affinity Chromatography on Protein G from tissue culture supernatant

Immunogen: Affinity purified Human Factor VIII

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:200 - 1:2000. Western blotting: 1:20 - 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Factor VIII

Target: Factor VIII (F8) (Coagulation Factor VIII (F8))

Background: Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and crosslinks to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries.Synonyms: AHF, Antihemophilic factor, F8C, Procoagulant component

Gene ID: 2157

NCBI Accession: NP_000123

UniProt: P00451