CD2 antibody

Catalog No. ABIN120363

Product Details anti-CD2 Antibody

Target: CD2

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the CD2 cell surface antigen, a 50-54 kDa glycoprotein expressed by thymocytes and mature T cells.

Purification: Affinity chromatography on Protein G

Immunogen: Activated rat T helper cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Clone: OX-34

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/50, use 10 μL of the suggested working dilution to label 106 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD2

Target: CD2

Alternative Name: CD2 (CD2 Products)

Background: CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95 % of thymocytes, it is also found on some natural killer cells but not on B lymphocytes. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggering of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukaemias of T cell origin.Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5

Gene ID: 497761

NCBI Accession: NP_036962

UniProt: P08921