CD3D antibody (Biotin)

Catalog No. ABIN120368

The Mouse Monoclonal anti-CD3D antibody has been validated for FACS. It is suitable to detect CD3D in samples from Rat.

Quick Overview for CD3D antibody (Biotin) (ABIN120368)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3D antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 1F4

Product Details anti-CD3D Antibody (Biotin)

Specificity: This antibody recognises CD3, a 25 kDa antigen which is found on rat T-cells. This antibody does not react with Rat B cells. Functionally the addition of the antibody to a culture of Rat T cells induces the proliferation of T-cells in the presence of PMA (See Nicolls, M.G. et al. for details). We recommend the use of SM253A for this purpose.

Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3 delta Chain

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D

Gene ID: 25710

NCBI Accession: NP_037301

UniProt: P19377

Pathways: TCR Signaling, CXCR4-mediated Signaling Events