CD5 antibody

Catalog No. ABIN120387

Product Details anti-CD5 Antibody

Target: CD5

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the rat CD5 cell surface antigen. OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue.

Purification: Affinity chromatography on Protein G

Immunogen: Rat thymocyte glycoproteins. Spleen cells from an immunised BALB/c mouse were fused with cells from the NS1 mouse myeloma cell line.

Clone: OX-19

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/25 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipiation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Background: CD5 is a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 54236

NCBI Accession: NP_062168

UniProt: P51882