CD31 antibody (FITC)

Catalog No. ABIN120451

This Mouse Monoclonal antibody specifically detects CD31 in FACS. It exhibits reactivity toward Rat and Rhesus Monkey.

Quick Overview for CD31 antibody (FITC) (ABIN120451)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Rat, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: TLD-3A12

Product Details anti-CD31 Antibody (FITC)

Specificity: This antibody recognises PECAM-1 (CD31), a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al. 2009). We recommend SM272LE for use in functional studies. This clone has been reported as suitable for use on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH 7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001).

Cross-Reactivity (Details): Species reactivity (expected):Rhesus Monkey.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G.

Immunogen: Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1

Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gene ID: 29583

NCBI Accession: NP_113779

UniProt: Q3SWT0

Pathways: Regulation of Actin Filament Polymerization