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SIRPA antibody (Biotin)

SIRPA Reactivity: Rat FACS Host: Mouse Monoclonal OX-41 Biotin

Catalog No. ABIN120468

Datasheet as PDF

Target See all SIRPA Antibodies

SIRPA (Signal-Regulatory Protein alpha (SIRPA))
Reactivity
76

39

34

10

9

3

3

3
Rat
Host
61

38

1

1
Mouse
Compatible Secondaries
Clonality
56

44

1
Monoclonal
Conjugate
56

10

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SIRPA antibody is conjugated to Biotin
Application
62

51

23

22

18

16

12

11

10

2

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises rat CD172a, also known as Signal regulatory protein (SIRP). MRC OX-41 binds to a different epitope on SIRP than ED9.

Immunogen

Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.

Clone

OX-41

Isotype

IgG2a

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SIRPA Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

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SIRPA Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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SIRPA Reactivity: Human, Rat, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

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SIRPA Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated

anti-Signal-Regulatory Protein alpha (SIRPA) (AA 105-324) antibody
SIRPA Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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SIRPA Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Signal-Regulatory Protein alpha (SIRPA) antibody
SIRPA Reactivity: Human FACS, IHC (fro) Host: Mouse Monoclonal 15-414 unconjugated

anti-Signal-Regulatory Protein alpha (SIRPA) (AA 151-250) antibody
SIRPA Reactivity: Human, Mouse WB, FACS, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry. Immunohistochemistry on frozen and paraffin sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

Containing 0.09 % Sodium Azide and 1.0 % Bovine Serum Albumin

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name

CD172a / SIRPA (SIRPA Products)

Background

CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Gene ID

25528

NCBI Accession

NP_037148

UniProt

P97710