CD44 antibody

Catalog No. ABIN120596

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, WB, IHC (p) and IP. It is suitable to detect CD44 in samples from Human, Dog and Pig.

Quick Overview for CD44 antibody (ABIN120596)

Target: CD44

Reactivity: Human, Dog, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: MEM-263

Product Details anti-CD44 Antibody

Specificity: The antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.

Purification: Protein A affinity chromatography

Immunogen: COS-7 cells (African Green Monkey)

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western Blotting: recommended dilution 2 mg/mL, 60 min on vertical incubator Positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6 % separating gel). Immunohistochemistry (paraffin sections) Recommended dilution: 10 m g/mL Positive tissue: uterus, myometrium
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for 1 month. For long-term storage aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling