CD52 antibody (PE)

Catalog No. ABIN120667

Product Details anti-CD52 Antibody (PE)

Target: CD52

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD52 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Human CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety, mature CD52 Molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, Monocytes/Macrophages and in male reproductive tract.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Size-Exclusion Chromatography

Immunogen: Human tonsil

Clone: HI186

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (use 20 μL to label 10^6 cells in a suspension or 100 μL whole blood).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade protease free BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1 (CD52 Products)

Background: CD52 is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

UniProt: P31358