For best experience we recommend to activate Javascript in your browser.

CD33 antibody

CD33 Reactivity: Human FACS Host: Mouse Monoclonal HIM3-4 unconjugated

Catalog No. ABIN120672

Datasheet as PDF

Target See all CD33 Antibodies

CD33
Reactivity
165

35

14

13

7

6

3

1
Human
Host
94

75

2
Mouse
Compatible Secondaries
Clonality
90

80

1
Monoclonal
Conjugate
63

14

13

12

9

6

5

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD33 antibody is un-conjugated
Application
106

101

42

33

26

26

23

22

17

14

9

9

6

5

4

2

2

2

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Purification

Protein-G affinity chromatography

Purity

> 95 % (by SDS-PAGE)

Immunogen

NFMY-9s human cell line

Clone

HIM3-4

Isotype

IgG1

Top Product

Discover our top product CD33 Primary Antibody
anti-CD33 (CD33) (AA 101-200) antibody
CD33 Reactivity: Human, Mouse FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 15-237) antibody
CD33 Reactivity: Human FACS, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated

anti-CD33 (CD33) (AA 15-237) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated

anti-CD33 (CD33) (AA 18-240) antibody
CD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 49-259) antibody
CD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 261-364) antibody
CD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD33 (CD33) (AA 1-364) antibody
CD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody
CD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated

anti-CD33 (CD33) antibody (Biotin)
CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin

Application Notes

Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH ~7.4, 15 mM Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date

12 months
Target

CD33

Alternative Name

CD33 / SIGLEC3 (CD33 Products)

Background

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

Gene ID

945

UniProt

P20138