alpha Fetoprotein antibody

Catalog No. ABIN120681

The Mouse Monoclonal anti-alpha Fetoprotein antibody (Clone AFP-01) (ABIN120681) specifically detects alpha Fetoprotein in WB, IP and EIA. The antibody is reactive with Human samples.

Quick Overview for alpha Fetoprotein antibody (ABIN120681)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: AFP-01

Product Details anti-alpha Fetoprotein Antibody

Specificity: The antibody reacts with 70 kDa alpha-Fetoprotein (AFP).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Precipitation Methods

Immunogen: Purified alpha-Fetoprotein

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. ELISA: The antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with clone AFP-11 (cat. no. ABIN120683). Western Blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both non-reducing and reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Background: AFP is an oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process