KIF5B antibody

Catalog No. ABIN120715

This Mouse Monoclonal antibody specifically detects KIF5B in IF. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for KIF5B antibody (ABIN120715)

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIF5B antibody is un-conjugated

Application: Immunofluorescence (IF)

Clone: KN-02

Product Details anti-KIF5B Antibody

Specificity: The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Purification: Thiophilic adsorption-affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Enriched fraction of porcine brain kinesin

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Alternative Name: KIF5B

Background: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.Synonyms: Conventional kinesin heavy chain, KIF5B, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Ubiquitous kinesin heavy chain

Gene ID: 3799

UniProt: P33176

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization