CD3 antibody

Catalog No. ABIN121123

This Mouse Monoclonal antibody specifically detects CD3 in FACS, IP and IHC (fro). It exhibits reactivity toward Chicken.

Quick Overview for CD3 antibody (ABIN121123)

Target: CD3

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CT-3

Product Details anti-CD3 Antibody

Purification: Ion Exchange Chromatography

Immunogen: Chicken thymocytes and Ig -ve peripheral blood mononuclear cells. Lymph node cells from immunised BALB/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1:50, Use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Borate buffered saline, None, None

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: In the chicken, CD3 is expressed by approximately 30 % of thymocytes, 70 % of peripheral blood mononuclear cells, but less than 1 % of cells in the bursa and bone marrow.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway