Endonuclease G antibody (AA 55-70)

Catalog No. ABIN121687

Product Details anti-Endonuclease G Antibody

Target: Endonuclease G (ENDOG)

Binding Specificity: AA 55-70

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 55 - 70 of endonuclease G. AA Sequence: GGPRGPGELAKYGLP

Application Details

Application Notes: Western Blot: 1 μg/mL - 2 μg/mL, this antibody detects a band of approximately 35 kDa in HepG2 and 3T3 cell lysates. Immunohistochemistry on paraffin sections: 15 μg/mL, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: Endonuclease G (ENDOG Products)

Background: Endonuclease G is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. endoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria.Synonyms: ENDOG

UniProt: Q14249

Pathways: Apoptosis