CCL24 antibody

Catalog No. ABIN121823

The Goat Polyclonal anti-CCL24 antibody is suitable to detect CCL24 in samples from Mouse. It has been validated for WB, EIA and Func.

Quick Overview for CCL24 antibody (ABIN121823)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL24 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL24 Antibody

Specificity: This antibody detects Eotaxin-2.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant mouse Eotaxin-2

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0 - 8.0 μg/mL of this antibody is required. ELISA: Indirect: To detect mEotaxin-2 (using 100 m l/well antibody solution) a concentration of 0.5 - 2.0 m g/mL of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2. Sandwich: To detect mEotaxin-2 (using 100 m l/well antibody solution) a concentration of 0.5 - 2.0 m g/mL of this antibody is required. In conjunction with Biotinylated Anti-Murine Eotaxin-2 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2. Western blot: To detect mEotaxin-2 this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeksat2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeatedfreezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Eotaxin-2 / CCL24

Background: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.Synonyms: C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, MPIF-2, MPIF2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24

Gene ID: 56221

UniProt: Q9JKC0

Pathways: Regulation of Actin Filament Polymerization