B-Cell Linker antibody

Catalog No. ABIN121944

The Rabbit Polyclonal anti-B-Cell Linker antibody (ABIN121944) specifically detects B-Cell Linker in WB. The antibody is reactive with Mouse samples.

Quick Overview for B-Cell Linker antibody (ABIN121944)

Target: B-Cell Linker (BLNK)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-B-Cell Linker Antibody

Specificity: The antibody reacts with BLNK, a cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK).

Purification: Precipitation methods

Purity: > 95 % (by SDS-PAGE)

Immunogen: Fusion protein of human BLNK AA Sequence: 171-356

Application Details

Application Notes: Western Blot: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for 1 month or aliquot and store at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: B-Cell Linker Protein / BLNK

Background: SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa, B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase Cg2, as well as Grb2 and Nck adaptor proteins, thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.Synonyms: B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa

UniProt: Q9QUN3

Pathways: BCR Signaling