GFP antibody (Green Fluorescent Protein) Primary Antibody
- Aequorea victoria
- This GFP antibody is un-conjugated
- Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
- The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.
- Affinity Chromatography on Protein A
- EGFP, a native full-length protein
- Alternative Name
- Target Type
- Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.Synonyms: GFP-Tag, Green fluorescent protein
- Application Notes
- Immunoprecipitation (10-20 μg/sample). Immunocytochemistry (1-3 μg/mL). Western Blotting (0.5-1.5 μg/mL). Positive control: transfected cells. Negative control: non-transfected cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
- For Research Use only
- 1.0 mg/mL
- PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (protease free)
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C/-20 °C
- Storage Comment
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
- Expiry Date
- 12 months
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- Prescher, Baumgärtel, Ivanchenko, Torrano, Bräuchle, Müller, Lamb: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, 2016 (PubMed).