CXCR4 antibody (Cytoplasmic Domain)

Catalog No. ABIN121952

The Rabbit Polyclonal anti-CXCR4 antibody has been validated for IHC (p). It is suitable to detect CXCR4 in samples from Human, Mouse and Rat.

Quick Overview for CXCR4 antibody (Cytoplasmic Domain) (ABIN121952)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCR4 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: This antibody Detects human CXC Chemokine Receptor 4 (CXCR4, Family: GPCR, Subfamily: Chemokine).

Purification: Peptide Immunogen Affinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: 9-14 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 / CXCR4

Background: CXCR4, a Chemokine Receptor involved in organ vascularization, neuronal cell migration, and patterning of the central nervous system during development. It binds stromal cell-derived factor 1 (SDF1, also called PBFS) and mediates migration of resting leukocytes and is unique in homing hematopoietic progenitors to bone marrow. These properties suggest that CXCR4 is involved in tumor cell migration and local tumor invasion. It is also an HIV-1 fusion co-factor that allows HIV-1 invasion in diverse human cell types. Although this receptor was initially called Neuropeptide Y3 Receptor, it does not respond to neuropeptide Y. CXCR4 has two isoforms that are produced by alternative splicing. CXCR4 has been reported to be expressed in cells of the immune and central nervous systems. It is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells. In brain tumors, CXCR4 is expressed in regions of angiogenesis and degenerative, necrotic, and microcystic changes. ESTs have been isolated from a wide variety of tissue libraries, particularly uterus, kidney, lung, tonsil and brain.Synonyms: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, NPYRL, SDF1 receptor, Stromal cell-derived factor 1 receptor

Gene ID: 7852

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events