CHRM3 antibody (C-Term)

Catalog No. ABIN122325

This anti-CHRM3 antibody is a Rabbit Polyclonal antibody detecting CHRM3 in IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for CHRM3 antibody (C-Term) (ABIN122325)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRM3 Antibody

Binding Specificity: C-Term

Specificity: Reacts with Muscarinic Acetylcholine Receptor M3 (Family: GPCR, Subfamily: Acetylcholine)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Peptide Immunogen Affinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 72 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7 containing 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: Muscarinic Acetylcholine Receptor M3

Background: Muscarinic acetylcholine receptor 3 is an Acetylcholine Receptor that controls smooth muscle contraction by mediating phosphoinositide hydrolysis and Ca2+ mobilization. Activation of this receptor causes secretion in glandular tissues. Mouse studies suggest that the M3 receptor may play a role in appetite stimulation and food intake. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3

Gene ID: 1131

UniProt: P20309

Pathways: Synaptic Membrane