PrxQ antibody

Catalog No. ABIN125852

The Rabbit Polyclonal anti-PrxQ antibody has been validated for WB. It is suitable to detect PrxQ in samples from Arabidopsis thaliana, Manihot esculenta, Spinach and Zea mays. There are 3+ publications available.

Quick Overview for PrxQ antibody (ABIN125852)

Target: PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))

Reactivity: Arabidopsis thaliana, Manihot esculenta, Spinach, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-PrxQ Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Populus spp. , monocots including Triticum aestivum, Oryza sativa

Characteristics: Expected / apparent Molecular Weight of the Antigene: 16 kDA

Purification: serum

Immunogen: His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060

Application Details

Application Notes: 1: 5000 with standard ECL (WB)

Comment:

In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-PrxQ antibody (ABIN125852)

Wu, Han, Wang, Wang, Sun, Zu, Chen: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, (2013) (PubMed).

Pulido, Spuinola, Kirchsteiger, Guinea, Pascual, Sahrawy, Sandalio, Dietz, Gonzualez, Cejudo: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." in: Journal of experimental botany, (2010) (PubMed).

Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).

Target Details for PrxQ

Target: PrxQ (PRXQ) (Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ))

Alternative Name: PrxQ

Background: AGI Code: At3g26060
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.

Molecular Weight: 16 kDA

UniProt: Q9LU86