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Psal antibody

This Rabbit Polyclonal antibody specifically detects Psal in WB. It exhibits reactivity toward Arabidopsis thaliana, Barley, Nicotiana tabacum and Spinach.
Catalog No. ABIN125860

Quick Overview for Psal antibody (ABIN125860)

Target

Psal (psi-L Subunit of Photosystem I (Psal))

Reactivity

Arabidopsis thaliana, Barley, Nicotiana tabacum, Spinach

Host

Rabbit

Clonality

Polyclonal

Application

Western Blotting (WB)
  • Cross-Reactivity (Details)

    Not reactive in: cyanobacteria

    Predicted Reactivity

    dicots and monocots (target sequence is only weakly conserved in Oryza sativa)

    Characteristics

    Expected / apparent Molecular Weight of the Antigene: 18 / 17-18 for Arabidopsis thaliana

    Purification

    serum

    Immunogen

    KLH-conjugated synthetic peptide derived from PsaL protein sequence from Arabidopsis thaliana (At4g12800). This sequence is well conserved in most mono- and dicots but not in Physcomitrella patens.
  • Application Notes

    1: 1000 with standard ECL (WB)

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 200 μL of sterile water

    Handling Advice

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target

    Psal (psi-L Subunit of Photosystem I (Psal))

    Background

    AGI Code: At4g12800
    PsaL (PSI-L) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Psa-L is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.In plants and algae Psa-L is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

    Molecular Weight

    expected: 18 kDa, apparent: 17-18 for Arabidopsis thaliana

    UniProt

    Q9SUI4
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