Vitellogenin 2 antibody
Quick Overview for Vitellogenin 2 antibody (ABIN125873)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Purpose
- Anti-VTG | Sole vitellogenin
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Cross-Reactivity (Details)
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Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Purification
- Serum
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Immunogen
- Native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)
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Application Notes
- 1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)
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Comment
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the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 μL of sterile water
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Buffer
- Serum
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Vitellogenin 2
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Alternative Name
- VTG
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Background
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Background: Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.
Additional information: VTG can be purified using following methodology: Mañanós et al. (1994). Sea bass (Dicentrarchus labrax L.) vitellogenin. I-Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2): 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.
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Molecular Weight
- ca. 200 kDa
Target
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