Vitellogenin 2 antibody
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- Target
- Vitellogenin 2
- Reactivity
- Sea bass (Dicentrarchus labrax)
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Predicted Reactivity
- n. a.
- Purification
- serum
- Immunogen
- native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)
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- Application Notes
- Recommended Dilution: 1 : 5 000 (WB), 1 : 5 000 on sole serum (ELISA).
- Comment
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the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µL of sterile water
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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- Target
- Vitellogenin 2
- Alternative Name
- VTG
- Synonyms
- VIT2 antibody, vit antibody, vitellogenin-2 antibody, vitellogenin 2 antibody, VTG2 antibody
- Background
- Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.
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