Vitellogenin 2 antibody

Catalog No. ABIN125873

Product Details

Target: Vitellogenin 2

Reactivity: Sea bass (Dicentrarchus labrax)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Predicted Reactivity: n. a.

Purification: serum

Immunogen: native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

Application Details

Application Notes: Recommended Dilution: 1 : 5 000 (WB), 1 : 5 000 on sole serum (ELISA).

Comment:

the developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: Vitellogenin 2

Alternative Name: VTG

Synonyms: VIT2 antibody, vit antibody, vitellogenin-2 antibody, vitellogenin 2 antibody, VTG2 antibody

Background: Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.