Cyt C6 antibody

Catalog No. ABIN125902

This Rabbit Polyclonal antibody specifically detects Cyt C6 in WB. It exhibits reactivity toward Chlamydomonas reinhardtii.

Quick Overview for Cyt C6 antibody (ABIN125902)

Target: Cyt C6 (Thylakoid Lumen Cytchrome C6 Protein (Cyt C6))

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Cyt C6 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata

Characteristics: Expected / apparent Molecular Weight of the Antigene: 15 kDa

Purification: serum

Immunogen: GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

Application Details

Application Notes: 1 : 1 000 with alkaline phosphatase (WB)

Comment:

Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Cyt C6

Target: Cyt C6 (Thylakoid Lumen Cytchrome C6 Protein (Cyt C6))

Background: Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.. Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

Molecular Weight: 15 kDa

UniProt: A8J9G6