LRP1 antibody

Catalog No. ABIN125951

The Mouse Monoclonal anti-LRP1 antibody has been validated for WB, FACS, IF and IHC (fro). It is suitable to detect LRP1 in samples from Human, Mouse, Rat, Rabbit and Hamster.

Quick Overview for LRP1 antibody (ABIN125951)

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Reactivity: Human, Mouse, Rat, Rabbit, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5A6

Product Details anti-LRP1 Antibody

Specificity: This monoclonal antibody 5A6 specifically reacts with the 85 kDa beta-chain of LRP/alpha2MR which is expressed in a restricted spectrum of cell types (for a recent review cf. Herz & Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. The antibody can also be used for the characterization of a subset of myelomonocytic subtypes of chronic and acute leukemia (CD 91). Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRP.

Purification: Affinity Chromatography on Protein A.

Immunogen: Human LRP/alpha2MR.

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen Sections (Dilute 1/20). Flow Cytometry. Immunoblotting. Neutralizing effects of 5A6 have also been reported (cf. Basu et al. , 2001).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water

Buffer: Final solution contains 0.5 % BSA in PBS buffer, pH 7.4

Precaution of Use: Do Not Add Sodium Azide

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for LRP1

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Alternative Name: CD91 / LRP1

Background: Synonyms: A2MR, APOER, APR, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, Prolow-density lipoprotein receptor-related protein 1

Gene ID: 4035

NCBI Accession: NP_002323

UniProt: Q07954

Pathways: Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling