Lamin B1 antibody

Catalog No. ABIN126714

This anti-Lamin B1 antibody is a Mouse Monoclonal antibody detecting Lamin B1 in WB, IF and IHC (fro). Suitable for Human, Mouse, Rat, Cow, Xenopus laevis and Fish.

Quick Overview for Lamin B1 antibody (ABIN126714)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat, Cow, Xenopus laevis, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X223

Product Details anti-Lamin B1 Antibody

Specificity: The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Lamin isotypes of Mr 60-75 kDa. Xenopus laevis LII, LIII Bovine LB1, B2 Mouse LB2 Rat + Human LB1, B2 Trout LII, LIII Rat kangaroo LII, LIII

Purification: Protein A affinity chromatography.

Immunogen: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence microscopy. Immunoblotting. Immunohistochemistry: 1/10 for 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water.

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin-B1 (LMNB1)

Background: Synonyms: LMN2, LMNB, LMNB1, LMNB2, Lamin B1/B2

Gene ID: 4001

NCBI Accession: NP_005564

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis