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beta Amyloid antibody (C-Term, N-Term)

Abeta Reactivity: Human WB, IF, EIA Host: Mouse Monoclonal 5C3 unconjugated
Catalog No. ABIN126719
  • Target See all beta Amyloid (Abeta) Antibodies
    beta Amyloid (Abeta) (Amyloid beta (Abeta))
    Binding Specificity
    • 16
    • 12
    • 10
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-40, C-Term, N-Term
    Reactivity
    • 70
    • 20
    • 14
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 38
    • 24
    • 6
    • 2
    • 2
    Mouse
    Clonality
    • 40
    • 25
    • 3
    Monoclonal
    Conjugate
    • 62
    • 5
    • 2
    • 1
    • 1
    • 1
    This beta Amyloid antibody is un-conjugated
    Application
    • 48
    • 47
    • 30
    • 17
    • 16
    • 12
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Specificity
    This antibody specifically interacts with the C-Terminus of beta-Amyloid (1-40) and does not crossreact with beta-Amyloid (1-42).
    Purification
    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
    Immunogen
    C-terminal peptide conjugated to KLH
    Clone
    5C3
    Isotype
    IgG1
    Top Product
    Discover our top product Abeta Primary Antibody
  • Application Notes
    Western blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunocytochemistry: 1 - 10 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mL2 x PBS/0.09 % Sodium Azide/PEG and Sucrose
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    beta Amyloid (Abeta) (Amyloid beta (Abeta))
    Alternative Name
    Amyloid beta (Abeta Products)
    Background
    The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).Synonyms: Amyloid beta peptide
    Pathways
    Inflammasome
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