CTNNA1 antibody

Catalog No. ABIN126738

This anti-CTNNA1 antibody is a Mouse Monoclonal antibody detecting CTNNA1 in WB, IF, IP and EIA. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for CTNNA1 antibody (ABIN126738)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D9

Product Details anti-CTNNA1 Antibody

Specificity: This antibody specifically recognizes α-catenin in Western blot at 102 kDa.

Characteristics: Synonyms: Alpha E-catenin, Alpha Catenin, Cadherin-associated protein, NY-REN-13 antigen

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Recombinant α-catenin

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1- 10 g per 10^6 vanadate-treated A431 cells. Immunocytochemistry: 0.1-1 μg/mL , mab a-CAT-15D9 may tolerate formaldehyd.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: Catenin alpha-1 / CTNNA1

Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The N-terminus of α-catenin directly interacts with exon 3 of β-catenin. The C-terminus of α-catenin interacts with the actin filament network.Synonyms: Alpha Catenin, Alpha E-catenin, Cadherin-associated protein, NY-REN-13 antigen

Gene ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location