CK1 epsilon antibody (AA 355-380)
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- Target See all CK1 epsilon (CSNK1E) Antibodies
- CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
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Binding Specificity
- AA 355-380
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CK1 epsilon antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.
- Characteristics
- Synonyms: CKI-epsilon, CKIe, CSNK1E
- Purification
- Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
- Components
- incl. positive Control
- Immunogen
- Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin
- Clone
- 4D7
- Isotype
- IgG1
- Top Product
- Discover our top product CSNK1E Primary Antibody
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anti-Casein Kinase 1, epsilon (CSNK1E) (C-Term) antibody
Verified CSNK1E Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) antibodyCSNK1E Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (Internal Region) antibodyCSNK1E Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 17-231), (Isoform epsilon) antibodyCSNK1E Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 1-416), (Isoform epsilon) antibodyCSNK1E Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) antibodyCSNK1E Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 331-360), (C-Term) antibodyCSNK1E Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB0845 unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 2-100) antibodyCSNK1E Reactivity: Human ELISA, IF, PLA Host: Mouse Monoclonal 2E1 unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 276-325) antibodyCSNK1E Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (Center) antibodyCSNK1E Reactivity: Human, Mouse, Rat WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- For positive control cell lysate from serum starved A431 cells.
- Restrictions
- For Research Use only
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- Reconstitution
- Reconstitute with 1 mL H2O (15 min, RT)
- Buffer
- 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
- Alternative Name
- Casein Kinase I epsilon (CSNK1E Products)
- Background
- Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E
- Gene ID
- 1454
- UniProt
- P49674
- Pathways
- Hedgehog Signaling, M Phase
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