MAPK7 antibody (N-Term)

Catalog No. ABIN126797

The Mouse Monoclonal anti-MAPK7 antibody has been validated for WB. It is suitable to detect MAPK7 in samples from Human, Mouse, Rat and Dog.

Quick Overview for MAPK7 antibody (N-Term) (ABIN126797)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPK7 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 12F2

Product Details anti-MAPK7 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes MAP kinase 7 (erk5) at 92 kDa in Western-blot applications.

Characteristics: Synonyms: Mitogen-activated protein kinase 7, Extracellular signal-regulated kinase 5, ERK4, Big MAPkinase 1, MAPK7

Purification: Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin. AA Sequence: N-Terminus

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive control: Cell lysate from untreated SKOV-3 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Alternative Name: MAPK7 / ERK5

Background: Erk5 (also known as MAPK7 or big mitogen activated kinase 1, BMK1) belongs to the Ser/Thr protein kinase family. It contains a T E Y motif in the activation loop similar to Erk1/2. Extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxdative stress lead to Erk5 activation by MEK5. The Erk5 pathway plays an important role in cellular proliferation, differentiation and survival.Synonyms: Big MAP kinase 1, ERK4, Extracellular signal-regulated kinase 5, MAPK7, Mitogen-activated protein kinase 7

Gene ID: 5598

UniProt: Q13164

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling