Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL H2O (15 min, RT).
Buffer
1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C/-80 °C
Storage Comment
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
Expiry Date
12 months
Target
GST
(Glutathione S Transferase (GST))
Alternative Name
Glutathione-S-Transferase (GST)
Background
The glutathione-S-transferase (GST) of Schistosoma japonicum is widely used as fusion partner in protein expression systems. The GST can be used for affinity purification of fusion proteins on immobilized glutathion as well as tag sequence if antibodies specific for the expressed protein are not available.Synonyms: GST26-Tag, Glutathione S-transferase Tag