MAP2K5 antibody

Catalog No. ABIN126841

This Mouse Monoclonal antibody specifically detects MAP2K5 in WB. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for MAP2K5 antibody (ABIN126841)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2K5 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 14B5

Product Details anti-MAP2K5 Antibody

Specificity: This antibody reacts to MKK5.

Characteristics: Synonyms: MAP kinase kinase 5, MAPKK 5, MEK5, MKK5, Dual specificity mitogen-activated proteinkinase kinase 5

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to hemocyanin

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MAP2K5

Background: Mitogen-activated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK subfamily (MAP2K, MKK or MEKs). MKK5 specifically phosphorylates erk5/MAPK7 within the kinase activation loop pThr-E-pTyr motif. After activation erk5 phosphorylates MKK5 on multiple phosphoserine residues. The MKK5/erk5 pathway may play an important role in prostate and breast cancer.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5

Gene ID: 5607

UniProt: Q13163

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway