Calreticulin antibody (C-Term)

Catalog No. ABIN126998

The Rabbit Polyclonal anti-Calreticulin antibody is suitable to detect Calreticulin in samples from Human, Mouse, Rat and Monkey. It has been validated for WB and IHC (p).

Quick Overview for Calreticulin antibody (C-Term) (ABIN126998)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calreticulin Antibody

Binding Specificity: C-Term

Specificity: Recognizes a 60 kDa calreticulin on SDS-PAGE immunoblots.

Purification: Protein A purified

Immunogen: Synthetic peptide from the C-terminus of Human Calreticulin

Application Details

Application Notes: Western blot: 0.5-2 μg/mL. Immunohistochemistry. NIH/3T3 cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 1 mg/mL BSA, 1.5 mM sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin

Background: Calreticulin is a calcium binding protein found in abundance in the endoplasmic reticulum and the sarcoplasmic reticulum. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus. Calreticulin has also been detected in the nucleus and nuclear envelop. Recent studies suggest that this soluble ER protein has a multifunctional role as it appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. Studies also suggest its involvement in certain autoimmune diseases. Consistence with its multiple functions the calreticulin molecule appears to have a zonal character. The protein has both high and low affinity calcium binding sites.Synonyms: CALR, CRP55, CRTC, Calregulin, ERp60, HACBP, grp60

Gene ID: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response