HRK antibody

Catalog No. ABIN127035

The Rabbit Polyclonal anti-HRK antibody (ABIN127035) specifically detects HRK in WB. The antibody is reactive with Human samples.

Quick Overview for HRK antibody (ABIN127035)

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRK Antibody

Specificity: The antibody detects Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 50 of human HRK.

Application Details

Application Notes: Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HRK

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Alternative Name: Harakiri / HRK

Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.Synonyms: Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, BID3, Neuronal death protein DP5

Gene ID: 8739

NCBI Accession: NP_003797

UniProt: O00198