XIAP antibody

Catalog No. ABIN127043

The Rabbit Polyclonal anti-XIAP antibody has been validated for WB, IHC (p) and IP. It is suitable to detect XIAP in samples from Human, Mouse and Rat.

Quick Overview for XIAP antibody (ABIN127043)

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-XIAP Antibody

Specificity: The XIAP antibody detects ~53 kDa human XIAP.

Purification: Affinity purified

Immunogen: Synthetic peptide surrounding amino acid 230 of human XIAP

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunoprecipitation. Immunohistochemistry. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used aspositive controls.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: IAP3 / BIRC4

Background: BIRC-4 (for baculoviral IAP repeat-containing protein 4) is a member of the IAP (for Inhibitor of apoptosis protein) family proteins. Other Aliases include RP1-315G1.5, API3, ILP1, MIHA, XIAP, XLP2. BIRC4 inhibits activated caspase-3 and apoptosis.Synonyms: API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, Inhibitor of apoptosis protein 3, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP

Gene ID: 331

NCBI Accession: NP_001158

UniProt: P98170

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis