Caspase 12 antibody

Catalog No. ABIN127076

The Rabbit Polyclonal anti-Caspase 12 antibody is suitable to detect Caspase 12 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Caspase 12 antibody (ABIN127076)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 12 Antibody

Specificity: The purified antibody detects Procaspase-12 (55 kDa) and cleavage Caspase-12 (36 kDa) on SDS-PAGE Immunoblots.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 310 of Mouse Caspase-12

Application Details

Application Notes: Western Blot: 0.5-4.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12

Background: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER, but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium hemeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-Secretase presenilin and beta-Amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.Synonyms: CASP-12, CASP12, Inactive caspase-12

Gene ID: 12364

NCBI Accession: NP_033938

UniProt: O08736

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response